Nitrate Reduction

Procedure 1
Prepare a dense “milky” suspension (at least McFarland No. 4) from the strain to be tested in 0.25 ml saline in a tube. Add one Nitrate Reduction tablet and close the tube. Incubate at 35-37 °C for 4 hours or 18-24 hours.

After incubation add 1 drop of N,N-Dimethyl-α-Naphthylamine sol. (Nitrate B Reagent) and 1 drop Sulfanilic acid sol. (Nitrate A Reagent). Read within 2 minutes.

Reading of the test
Positive reaction: Red/pink
Negative reaction: Colourless, light pink

Procedure 2
When testing anaerobes, the tablet can also be placed on an inoculated plate, which is incubated for 24-48 hours.

After incubation 1 drop each of N,N-Dimethyl-α-Naphthylamine sol. (Nitrate B Reagent) and Sulfanilic acid sol. (Nitrate A Reagent) is added to the tablet.

Reading of the test
A pink or red colour is interpreted as positive indicating reduction of nitrate to nitrite.

 

Specifications

  • Quantity: 50 tablets
  • Product Code: 437-11

Related Products

  • Nitrate Reagent A

    Nitrate Reagent A

    Intended Use
    The nitrate test is used to demonstrate the ability of the test organism to reduce nitrate to nitrite and to reduce nitrite to nitrogen.

  • Nitrate Reagent B

    Nitrate Reagent B

    Intended Use
    The nitrate test is used to demonstrate the ability of the test organism to reduce nitrate to nitrite and to reduce nitrite to nitrogen.

     

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